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PCB 4023 Cell Staining Question

 

  •  Write down the question being addressed by the experiment (1.5 points).

3.)  Create  a  summary  of  the  overall approach you use  to  answer  the  question  being addressed.Do not confuse this section with a material and methods section (1.5 points)

4.) Account for the one by one steps of the method that was used.The easiest way to do that  is by writing “see protocol listed in _______” citing an  online  resource,  specific pages  from  a lab  manual, or  a  published research  paper.  Also,  be  sure  to  note  whether anything you  did deviated  from  that  standard  protocol  by including comments like “We followed the protocol as described except for step 2 where we did _______ instead,” etc. Also, be sure to record any technical errors that occur while doing the procedures, as this saves a lot of confusion later (0.5 points).

5.)  Accurately  record and  show all results yielded  by  the  experiment(raw  data)as the data become available. Based  on  the  raw  results  obtained  (as  a  class),  prepare  an informative graph or table;  you may  choose one  or the other (or both) depending on the experiment and depending  which is the most efficient way to deliver the information.If you get pictures on the microscope, print them out and tape them into the notebook (or if you  cannot,  try  to  briefly  sketch  what  you  saw)(1.0  point). Write  down  a  paragraph  or two describing the results that  you obtained (as a class).Make sure NOT to include any discussion, just plain straight results and also to write down notes about anything unusual you see(1.0 point).

6.)   After   you   have analyzed the   data,   write   1-3   sentences that summarize your interpretation and explanation of the  findings.  Note  that  this  interpretation  section  is the  experiment  and  not  about  what  you  have  learned  or  your  experience  in  the  lab;  it  is not  about  troubleshooting  the  experiment  either,  it  is  about  looking  for  a  scientific explanation for the results obtained (1.5 points).

7.)  Last,  include  a  statement  about possible future scientific directions using as  a  base your findings from the experiment.This is not a section to name the mistakes you did in the experiment; but rather to suggest a future experiment/s using as a base the knowledge obtained  in  the  experiment in  the  lab.  Make  sure  you  are  specific about proposing  the future experiment (1.5 points).

8.) Write here the answers to the questions included at the end of each manual (1 point)

 1.Describe the staining pattern obtained foractin for each of the two cell types tested.Were they the same? Explain.

 2.What types of cell staining did you use today in the lab? Assign each of the 3 stains used with atype of staining methodology.

 3.What type of immunostaining did you use today?Explain your answer.

 4.Name one advantageof using an indirect immunostaininginstead of a directone.