Science Homework Help

1. Watch the following video on antibiotic resistance: 60 minute special on antibiotic resistance
2. Watch the following TedEd video on antibiotic resistance: TedEd video discussing Antibiotic resistance
3. Watch the following educational video from Yale on Phage Therapy: Yale University lecture on Phage Therapy
4. Write a brief review – 200 words describing the primary points that have challenged your thinking based on these videos.

IV. Read the following handout from the American Society of Microbiology on testing antibiotics:

American Microbiology Society Kirby Bauer Disk Diffusion lab

V. Answer the following 10 questions and submit in the appropriate dropbox.

1. Define the Kirby Bauer Disc Diffusion Method?
2. What is the Purpose of the Kirby Bauer Disc diffusion Method for testing antibiotics?
3. Define a zone of inhibition?
4. Define Resistance to antibiotics?
5. Define Susceptibility to antibiotics?
6. Define an intermediate Kirby Bauer Disc diffusion result?
7. Why is the Mueller Hinton Agar used for the Kirby Bauer Disc Diffusion method?
8. What is a “confluent lawn of growth” and why is it important for this laboratory test?
9. Is a larger zone of inhibition (greater diameter) a definite indicator of greater susceptibility by the bacteria?  Explain based on the American Society for Microbiology Handout.
10. As an example, let’s say you plated Staphylococcus aureus on Mueller Hinton Agar and tested it’s susceptibility to the following antibiotics and results:
    1. Clindamycin                        zone of inhibition = 7 mm
    2. Oxacillin                               zone of inhibition = 14 mm
    3. Penicillin                              zone of inhibition = 22 mm
    4. Vancomycin                       zone of inhibition = 16 mm
    5. Gentamycin                       zone of inhibition = 20 mm

Based on these results, rank the results from susceptible to resistant.  Use the information provided in the American Society for Microbiology handout linked to this document.  Justify your answers.

               Ie.  Staphylococcus is most susceptible to……  2nd …….3rd…….. etc.